Differential expression statistics were used to define a gene weight vector u j (of size equal to the total number of genes in the considered analysis) for each cell type j combining the log fold change x ij of each gene i with its adjusted p -value (Benjamini-Hochberg method [ 69 ]); to reduce noise, scores associated with marginal significance were set to zero, that is y i j = − log 2 ( x i j ) log 1 0 ( p i j ) when p ij < 0.05 and log 2 ( x ij ) ≥ 0.5, while y ij = 0 otherwise.
1
—
—