an increasing trendP = 0.02
5 ): Escape Latency: + 10 Gz Group: Compared to the control group (N group: 8.60 ± 6.24 s), the escape latency was significantly prolonged after acceleration exposure (6 h: 99.96 ± 18.63 s; 24 h: 101.28 ± 17.33 s; 72 h: 111.88 ± 7.96 s; all P < 0.0001) and showed an increasing trend over time (6 h vs. 72 h, P = 0.02). 5-HTE + 10Gz Group: At the 6 h time point, the escape latency was significantly shorter than that of the + 10 Gz group (18.16 ± 2.71 s vs. 99.96 ± 18.63 s, P < 0.001), indicating that tryptophan supplementation improved spatial memory ability. 5-HTD + 10Gz Group (Fig. 6 ): At all time points (6/24/72 h), the rats failed to reach the platform (escape latency = 120 s), indicating that tryptophan depletion led to severe cognitive impairment.