Specificity of Primers and Probes for Molecular Diagnosis of Leishmania (Leishmania) chagasi in Dogs and Wild Animals.

Pathogens · 2025 · PMC12566895 · PMID 41156675

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highly significantno p-value reported

Specificity analysis using Primer-BLAST (NCBI) demonstrated that all oligonucleotides have 100% identity and coverage with the reference sequence of the Leishmania (L.) donovani/infantum kDNA minicircle (GenBank accession number: MT598578.1 ), with highly significant E-values and scores ( Table A3 ), confirming their suitability for molecular diagnostic applications.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.

Specificity of Primers and Probes for Molecular Diagnosis of &lt;i&gt;Leishmania&lt;/i&gt; (&lt;i&gt;Leishmania&lt;/i&gt;) &lt;i&gt;chagasi&lt;/i&gt; in Dogs and Wild Animals.

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Specificity of Primers and Probes for Molecular Diagnosis of <i>Leishmania</i> (<i>Leishmania</i>) <i>chagasi</i> in Dogs and Wild Animals.