Chick early amniotic fluid attenuates lipopolysaccharide-induced acute lung injury.

Given that NAD + , guanosine and deoxyinosine may be significant components of ceAF that may mimic the anti-inflammatory capacity of ceAF, we first conducted in vitro studies using CCK8 assays in two cell lines, including the human lung epithelial cell line (BEAS-2B) and the mouse macrophage (RAW264.7) cell lines, to identify the optimal doses ( Fig. 5A, B ).

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