Simultaneous detection of respiratory virus RNA on environmental surfaces in a university setting by a sensitive Surface 3-Step PCR platform.

Sci Rep · 2025 · PMC12583612 · PMID 41184518

Europe PMC DOI

hedged sentences

0.0199

closest p · 0.4× alpha

0.0199

boldest claim

The sentences

an increasing trendp = 0.0199actually significant

An increasing trend in Ct values for ORF1b/N (SARS CoV-2), M1/NS2 (Flu A/B), M (RSV A/B) was found from T1 to T3, reaching statistical significance for the SARS-CoV-2 RdRp ( p = 0.0199) (Fig. 2 ).

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a decreasing trendno p-value reported

However, the first step of the method applied in our work allowed to detect SARS-CoV-2 RNA in 9.5% of samples, with a decreasing trend during time intervals and a main prevalence on student desks.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.