highly significantP ≤ 0.001
Significance thresholds were defined as follows: P > 0.05 (not significant, ns); P ≤ 0.05 (significant, *); P ≤ 0.01 (highly significant, **); P ≤ 0.001 (very significant, ***); and P ≤ 0.0001 (extremely significant, ****).