SCAN: an automated phenotyping tool for real-time capture of leaf stomatal traits in canola.

Stomatal density showed a decreasing trend as the leaf expands, and was consistently higher on the abaxial leaf surface compared with the adaxial surface at all leaf positions ( Fig. 3A ).

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In contrast, stomatal size showed an increasing trend with leaf expansion, suggesting a negative relationship with stomatal density and pore area ( Fig. 3A ).

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