Phenylalanine Modification in Plasma-Driven Biocatalysis Revealed by Solvent Accessibility and Reactive Dynamics in Combination with Protein Mass Spectrometry.

Besides Met and Cys, the amino acids Phe, Arg, and Lys were chosen for the modification analysis because of their predicted strong interaction with ROS, such as OH (SASA interaction analysis). Initially, the predicted reactions with ROS were investigated experimentally. The mass spectrometric analysis showed highly significant oxidation of Phe51, Phe67, Phe208 (Phe+O), Met42, and Met220 (Met+O) in all three replicates after 5 min of plasma treatment ( Table ).

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