highly significantp < 0.0001
This difference was highly significant (one-way ANOVA: F = 98.53, p < 0.0001), and a post hoc Dunnett’s test confirmed significant differences between our SYNZIP2-GFP control and SpyTagged GFP (SYNZIP2-GFP vs GFP-SpyTag: t = 6.925, p < 0.0001; SYNZIP2-GFP vs SpyTag-GFP: t = 14.04, p < 0.0001).