Co-Analysis of Transcriptome and Metabolome Reveals Flavonoid Biosynthesis in <i>Macadamia Pericarp</i> Across Developmental Stages.

Further analysis revealed that these metabolites exhibited a decreasing trend during fruit development ( Figure 3 d).

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Here, we measured the total amino acid content in the pericarp and found that it peaked at the S2 stage, showing an overall trend of initial increase followed by a decrease, albeit with minor fluctuations.

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The content of isoflavones generally exhibited an increasing trend, reaching the highest level at the late developmental stage (S5), which was approximately 1.67 times that of S1 ( Figure 1 g).

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The upstream PAL genes (LOC122086992, LOC122058157, LOC122061804, LOC122061512, LOC122061515) showed highly significant positive correlations with most flavonoid compounds.

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