highly significantp < 0.001
However, the analysis did identify two specific conditions where the effect of time was statistically significant, demonstrating a reliable change in CFU over the measured period: Listerine 50% on Polyamide (PA) showed a highly significant effect of Time (F = 35.590, p < 0.001), confirming a strong and reliable time-dependent bactericidal action, and the change over time for peppermint oil on PMMA (acrylic) was also found to be statistically significant (F = 3.744, p = 0.047).