Metabolomics reveals pro-inflammatory effects of 12R-HETE and ALOX12B in maternal allergic asthma.

Next, we conducted an overexpression experiment of ALOX12B in 16HBE cells, and the qPCR and WB analysis showed that both the mRNA and protein levels of ALOX12B exhibited an increasing trend ( p < 0.05) (Figs. 5 C, D, and S 4 ); Targeted quantitative analysis of 16HBE cells overexpressing ALOX12B showed that the overexpression of ALOX12B significantly increased the level of 12R-HETE in the cells ( p < 0.05) (Fig. 5G ).

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The results showed that after knocking down ALOX12B, the mRNA and protein levels of ALOX12B in 16HBE cells did exhibit a decreasing trend ( p < 0.05) (Figs. 6 A, B, and S 5 ); The mRNA levels of inflammation-related factors GMCSF, IL-6, IL-33, and MUC5AC (Fig. 6C ) as well as their protein levels (Fig. 6D ) were significantly decreased ( p < 0.05).

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