Comparing alignment and de-novo approaches for gut microbiota metagenomic data analysis reveals differences in taxonomic resolution and novel functional insights.

Sci Rep · 2025 · PMC12660995 · PMID 41309890

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a decreasing trendno p-value reported

PERMANOVA identifies the BMI group as a significant factor in both approaches, and alpha diversity consistently shows a decreasing trend as BMI increases.

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