highly significantp < 0.0001
In sharp contrast to the lack of discernible directional preference in the horizontal chambers, we found that N2 dauer larvae demonstrated an enhanced and highly significant preference for upward migration in the vertical chambers under these conditions (average vertical location = +1.63, n = 4,358 worms, 12 trials; p < 0.0001, all comparisons) ( Figure 2 A and 2B).