Investigating insulin's role in regulating local aromatase in growth plate development.

Immunohistopathological analysis Immunohistochemistry results revealed that compared with the control group, the growth plates of obese rats showed higher expression of aromatase and strong positive insulin receptor expression in hypertrophic regions ( Figure 2 ).This was further confirmed by a highly significant difference in average OD values in the growth plates of the obese group compared to the control group. qPCR and western blot analysis of growth plates for detecting the expression of CYP19A1 and insulin receptors The total RNA purity at OD260/280 was 1.88–1.97 and OD260/230 was 1.94–2, indicating that purity was in the standard range.

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