Neonatal gut microbiota stratification and identification of SCFA-associated microbial subgroups using unsupervised clustering and machine learning classification.

Front Microbiol · 2025 · PMC12712340 · PMID 41425919

Europe PMC DOI

hedged sentences

0.0010

closest p · 0.0× alpha

0.0600

boldest claim

The sentences

highly significantp = 0.001actually significant

The PERMANOVA test again demonstrated highly significant differences among clusters ( p = 0.001) with higher explanatory power (R 2 = 0.358 for K-Means and R 2 = 0.343 for Agglomerative), suggesting that three-cluster solutions captured more refined structural variations within the microbial community.

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a trend toward significancep = 0.06so close (0.05 < p ≤ 0.1)

In K-Means clustering, propionate levels differed significantly ( p = 0.010), with acetate showing a trend toward significance ( p = 0.06), while butyrate differences were not statistically significant.

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