Single-cell transcriptomic profiling of zebrafish primary motor neurons reveals subtype-specific gene expression signatures.

We selected lmo1 , a cotranscription factor, and the TFs, cdx4 and mafa , for smiFISH validation based on their highly significant differential expression among pMN subtypes and their absence of known expression in neighboring tissues that could obscure the signal in pMNs, such as muscle cells.

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