Optimized Whole-Mount Fluorescence Staining Protocol for Pulmonary Toxicity Evaluation Using Mouse Respiratory Epithelia.

Methods Protoc · 2025 · PMC12736217 · PMID 41441189

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an apparent trendno p-value reported

Group data were then compared using two-way ANOVA and post hoc Šídák’s multiple comparisons test (Prism 10.3.0; GraphPad Software), which revealed that while there was an apparent trend for increased cell death at higher doses of H 2 O 2 , this only became significant for ciliated epithelial cells subjected to 1% H 2 O 2 .

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