Barely Significant
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Cell2Read: an automated workflow to generate sequencing-ready DNA libraries from human cell suspensions.

Biol Methods Protoc · 2025 · PMC12744386 · PMID 41467035

Europe PMCDOI
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hedged sentences
0.0001
closest p · 0.0× alpha
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boldest claim

The sentences

highly significantP < .0001actually significant
One-way ANOVA revealed a highly significant difference between groups [ F (5,16) = 86.47, P < .0001, R 2 = 0.96], with significant variance heterogeneity detected by Brown-Forsythe ( P < .0001).

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a numerical trendno p-value reported
Post hoc Šídák’s multiple comparisons revealed no significant differences at any specific input level (adjusted P > .05), despite a numerical trend toward higher DNA recovery with the manual method at higher inputs (e.g. 108.9 ng more at 50 000 cells).

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