highly significantP < 0.01
To test the specificity of our immunosensor for PHB1, we compared the sensor’s response separately to solutions containing only PHB1 or only a closely related, structurally similar isoform (PHB2). Statistical analysis (unpaired two-tailed t test) revealed a highly significant difference ( P < 0.01) in the frequency shifts between response to PHB1 and PHB2, confirming high target selectivity for PHB1, as illustrated in Figure C and supplementary Figure S2 .