EXPLANA: a user-friendly workflow for EXPLoratory ANAlysis and feature selection in cross-sectional and longitudinal microbiome studies.

Bioinformatics · 2026 · PMC12766912 · PMID 41416890

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an expected trendno p-value reported

In some designs, such as interventions, or some observational studies with an expected trend over time [e.g. gut microbiome changes over the first years of a baby’s life ( Bokulich et al. 2018 )], changes are expected to be compared to a baseline value, so Δs can be calculated using baseline as a reference ( Ferrocino et al. 2018 , Frey et al. 2022 ).

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