Observed differences in leaf discolouration were quantified using an average pixel intensity calculation applied to each leaf image using ImageJ (version 1.52a), with highly significant differences in the proportion of discoloured leaf tissue observed between WT Pto, hrcN G176A and hrcN G311A ( S1C Fig ).
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Cyclic-di-GMP controls Type III effector export and symptom development in Pseudomonas syringae infections via the export ATPase HrcN.
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