High-Resolution <sup>1</sup>H NMR Investigation of the Speciation Status of Nickel(II) and Copper(II) Ions in a Cell Culture Medium: Relevance to Their Toxicological Actions.

Significance Analysis of Metabolomics (SAMs) Involving 1 H NMR Bucket Variables For the Cu(II) titration data (featuring added levels of 0–67 μmol/L), this MV analysis yielded very low, very highly significant q values (i.e., very close to zero), and the rank Cu(II)-broadening order of these found was found to be isoleucine > β-glucose > lysine > acetate > valine > methionine > leucine > pyroglutamate > arginine > dimethylsulphone > threonine > methanol > aspartate > N-acetylsugars (q ≈ 0 for all of these), and cystine ( q = 0.002); a q value is equivalent to an FDR-corrected p value.

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