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The performance of different methods in characterizing soil live prokaryotic diversity and abundance is highly variable.

IMetaOmics · 2025 · PMC12806490 · PMID 41675150

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a decreasing trendno p-value reported
Specifically, the robustness of the networks showed a decreasing trend in the following order: rRNA‐based analysis, alkaline buffer washing, PMA treatment, DNase pre‐digestion, and total DNA extraction (Figure 3F ).

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