Multispectral imaging and automated analysis for quantifying grain quality to reveal known and potential novel alleles affecting grain traits in wheat.

Then, we assessed the automated analysis result of seed segmentation against manually counted seed number and seed object regions from 100 seed lots, obtaining a highly significant positive correlation (R 2 = 0.996, RMSE = 0.656, P -value < 0.001; mIoU = 0.947; Supplementary Figure S4 ).

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