Raman spectroscopy reveals growth phase-dependent molecular differences in bacterial membrane vesicles.

Figure 4D displayed the protein-to-lipid ratio by analyzing the relation of amide I intensity at 1,645 cm −1 to lipid-assigned C-H 2 twisting at 1,302 cm −1 and revealed highly significant differences in protein portion based on the lipid content between isolations at 4 and 8 h, 8 and 12 h, as well as 16 and 20 h.

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