Optimization of fermentation conditions and extracellular metabolic profiling of Streptomyces rochei strain W71.

Optimization by Box–Behnken (BB) design Using inoculum size (E), temperature (F), and initial pH (H) as independent variables and inhibition rate (Y) as the response, a quadratic regression model was obtained with Design-Expert 13.0: .ANOVA demonstrated that the regression model was highly significant (P < 0.0001), with an R2 of 0.9928, indicating that 99.28% of the variation could be explained by the model.

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