The qRT-PCR results showed a highly significant positive correlation with the RNA-seq data ( Supplementary Figure 3 ; Supplementary Table 2 ). 3.5 Integrated multi-evidence analysis identifies TraesCS6B03G1211800 as the causal candidate To robustly prioritize the causal gene from the 25 candidates and mitigate the risk of false negatives from expression screening alone, we employed a multi-layered evidence integration strategy.
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Fine mapping and candidate gene mining of major QTL <i>QSL.caas-6BL.1</i> for spike length in bread wheat (<i>Triticum aestivum</i> L.).
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