Metabolic engineering of Escherichia coli BW25113 for the production of Vitamin K<sub>2</sub> based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification.

J Biol Eng · 2026 · PMC12879462 · PMID 41507964

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a decreasing trendno p-value reported

However, the VK 2 titer of the other strains showed a decreasing trend at 48 h.

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Metabolic engineering of Escherichia coli BW25113 for the production of Vitamin K&lt;sub&gt;2&lt;/sub&gt; based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification.

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Metabolic engineering of Escherichia coli BW25113 for the production of Vitamin K<sub>2</sub> based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification.