highly significantp < 0.001
The results of the CCK-8 cell proliferation assay and senescence-associated SA-β-gal staining for the percentage of senescent cells demonstrated that H 2 O 2 -induced KGN cells exhibited significant proliferation inhibition Statistical analysis of CCK-8 data demonstrated highly significant main effects of time (F 4 , 60 = 329.4, p < 0.001) and dosage (F 5 , 60 = 349.9, p < 0.001), along with a significant dosage × time interaction (F 20 , 60 = 45.7, p < 0.001), indicating a marked decline in cell viability with prolonged incubation and enhanced inhibition at higher concentrations, which suggests a synergistic interaction ( Figure 2A ).