Identification of Key Genes Regulating Body Weight in Qingyuan Partridge Chickens During Development Using RNA-Sequence Analysis.

Quantitative Real-Time PCR (qPCR) Six key candidate genes ( CALM2 , CHRND , HSP90AA1 , SLC8A3 , CD9 , PENK ) were strategically selected for qPCR validation based on the following criteria: (1) they were identified as top hub genes in the WGCNA co-expression modules most significantly correlated with muscle growth traits; (2) they exhibited highly significant differential expression across developmental stages in RNA-seq analysis; and (3) they represent core biological pathways implicated in muscle development and metabolism.

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