Persistent and Long-Term Infectivity of Dengue Virus in Mosquito Cells Revealed Reduced Replication in Vector Host and Human Endothelial Cells.

Remarkably, we found that DENV2 loads measured by QRT-PCR analysis showed an increasing trend in naïve recipient mosquito cells incubated with virus supernatants from days 3 to 19; however, we noted a significant decrease in infection loads with virus supernatants from the day 33 to 175 timepoints ( Figure 4 A).

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