Variation in sperm performance and mitochondrial metabolism of Mytilus spp. from the North and Baltic Seas under different environmental scenarios.

In contrast, at lower osmolarities (150–270 mOsm), ROS efflux peaked at 20°C and showed a decreasing trend at 25°C, although the difference was not statistically significant ( Fig. 8A ).

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The ROS efflux of sperm mitochondria in the OXPHOS state showed an increasing trend with rising temperature in higher osmolarity treatments (390–630 mOsm).

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