highly significantp < 0.01
NK4A214_group showed a positive correlated with the numbers of total born and born alive ( p < 0.05), and a highly significant positive correlated with the numbers of healthy piglets ( p < 0.01).
NK4A214_group showed a positive correlated with the numbers of total born and born alive ( p < 0.05), and a highly significant positive correlated with the numbers of healthy piglets ( p < 0.01).
Dietary treatment also influenced the microbiota, showing a trend toward significance for Shannon diversity ( p = 0.051; Figures 2A , B ) and a significant effect on richness as estimated by Chao1 ( p = 0.038; Figures 2D , E ).
The latest study also confirmed that alpha diversity (observed ASVs) showed a significant increase or an increasing trend across different parities, from gestation day 0 to day 110 ( Liu et al., 2025 ).