We do agree with the reviewer that estimating the ratios of microsporidia vs host DNA in the samples through PCR is not an ideal approach, and here our only intent was to observe an overall trend of sample composition to confidently deploy DNA sequencing for an unbiased estimation of the sample purity.
← all excerpts
In vitro culture of human-infecting Encephalitozoon spp. for genome sequencing with minimal host contaminant.
1
—
—