A highly significant enrichment (probability of observing this proportion <0.0001) for pSymA was found in all the strains, both for duplicated and deleted/mutated genes (Figure 1 ). pSymB was also found to be enriched for duplicated ORFs, though not as significantly as pSymA, except in strain AK58 where pSymB was significantly enriched for duplicated ORFs (probability of observing this proportion <0.0001).
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Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains.
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