Circadian rhythm disruption impairs ovarian follicular development via NAD<sup>+</sup> metabolic reprogramming.

We reviewed literature published by our research group and other published article, which reported highly significant ( p < 0.05) effects in follicle counting and ovarian section staining. 27 , 28 Analysis of these data indicated that, with α = 0.05 and β = 0.8, the sample size required to detect similar effect sizes was estimated to be around 3 animals per group.

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The results showed that the LP + NMN group (5.50 ± 1.29) exhibited an increasing trend in litter size compared to the LP group (3.25 ± 2.50) ( p = 0.600, one-way ANOVA) ( Fig. 7 h and i).

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35 The expression levels of most enzymes in NAD + synthesis pathway showed a decreasing trend by qPCR ( Fig.

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