highly significantP < 0.001
Statistical analyses were performed using GraphPad Prism software, with significance levels defined as follows: ∗ P < 0.05 (significant), ∗∗ P < 0.01 (highly significant), ∗∗∗ P < 0.001 (extremely significant), and ∗∗∗∗ P < 0.0001 (most significant).