Energy metabolism and the transcriptome provide new insights into the response mechanism of Manila clam (Rudiatapes philippinarum) between Southern and Northern China populations under high-temperature stress.

Pearson correlation analysis between qRT-PCR (ΔΔCt method) and RNA-seq (log2FC) data revealed a highly significant positive correlation (Pearson’s r = 0.965, R2 = 0.984, 95% CI: 0.942–0.991, p < 0.0001), demonstrating strong consistency between the two platforms.

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