highly significantp ≤ 0.001
The differences with p > 0.05 were considered not significant (ns); 0.01 < p ≤ 0.05, significant ( * ); 0.001 < p ≤ 0.01, highly significant ( ** ); 0.0001 < p ≤ 0.001, very highly significant ( *** ); and p ≤ 0.0001, extremely significant ( **** ).