RSM-GA-BP optimization of Ultrasound-Enzyme-Assisted deep eutectic solvent extraction (UEADESE) for flavonoids from Abelmoschus manihot (L.) leaves and mechanistic insights.

As shown in Table 4 , the model P -value was < 0.0001, indicating a highly significant model, while the P -value for the lack-of-fit term was 0.0642 (> 0.05), suggesting that the lack-of-fit was not significant.

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Within the range of 20% to 30%, the extraction yield showed a positive trend, primarily resulting from a moderate viscosity reduction and increased polarity of the DES.

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