Optimization of fermentation conditions for cellulase/xylanase production and hydrolysis conditions for efficient conversion of agricultural residues using Penicillium oxalicum UNN1.

As the hydrolysis time increased, the yield of reducing sugars showed an increasing trend, and all reached a peak at 12–24 h with 0.306 g/g to 0.369 g/g SBX.

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We have previously reported that 5 mM Cu2+ exhibits a highly significant inhibitory effect on the activity of xylanase BsXynA (Zhang et al. 2025 ), which aligns with the findings of this study.

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