To determine the specificity of the sfGFP-2×dLBT design, we tested the fluorescence response of sfGFP-2×dLBT to calcium ions. Compared to the buffer control, a decreasing trend in the fluorescence intensity of the sfGFP-2×dLBT sensor was observed with the dosage of calcium ions.
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A Chimeric LBT-GFP Biosensor Exhibits Antithetical Fluorescence Responses to Ca<sup>2+</sup> and Dy<sup>3+</sup> Binding.
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