Effects of varying levels of valine supplementation on meat metabolism and rumen microorganisms in Tibetan sheep.

In the negative ion mode, when compared to K, there was an observed increase in organic acids and derivatives, as well as nucleosides, nucleotides, and analogs in V1 group, whereas organic oxygen compounds, phenylpropanoids and polyketides, and lipids and lipid-like molecules showed a decreasing trend ( Supplementary Figure S1A ); In the V2 group, benzenoids, organic heterocyclic compounds, organic oxygen compounds, organic acids and derivatives, and nucleosides, nucleotides, and analogs were upregulated, in contrast to the downregulation of lipids and lipid-like molecules ( Supplementary Figure S1B ).

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