Surprisingly, when targeting eight different genomic sites using an CRISPR array of eight crRNAs, 66.6% of cells integrated at three sites and 16.6% of cells integrated at five sites after one round of transposition (infection for 4 h), and the overall efficiency showed an increasing trend in passaging supplemented with phage suspension (Fig. 3c ).
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A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library.
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