Chromosome-scale genome assembly of <i>Naematelia sinensis</i> (Jin Er mushroom) offers new insights into the distinct mating-type genomes feature and genome evolution.

Gene family analysis highlighted frequent expansions and contractions throughout the evolutionary history of Naematelia , with an overall trend toward contraction.

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The distribution of adjusted p -values further illustrated functional enrichment significance differences: NS-58 exhibited the broadest significance range (approximately 2–16), encompassing multi-level functions from marginally significant to highly significant; NS-27 and NS-45 showed significance concentrated in the moderate-to-high range (approximately 4–12); while NS-29 displayed a relatively concentrated significance distribution ( Figure 7C ).

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