highly significantP < 0.01
Differences were considered statistically significant at P < 0.05, highly significant at P < 0.01, and extremely significant at P < 0.001. 3 Results 3.1 Phylogenetic Analysis and Protein Sequence Characteristics of SeCaspase-4 SeCaspase-4 was cloned from the embryonic cells of S. exigua (Se-3 cells) and analyzed based on the S. exigua genome, as described in our previous studies. , The full-length ORF of SeCaspase-4 was 1638 bp, encoding 545 amino acids.