A novel rapid detection approach based on CRISPR-Cas13a for Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acariformes: Pyroglyphidae).

The total volume of qPCR reaction was 25 μl, containing 12 μl of Hieff qPCR SYBR Green Master Mix (Lesun Biotechnology Co., Ltd., Wuxi, China), 1 μl of plasmid (10 5 copies/μl), 2 μl each of upstream and downstream primers (10 μM/l), and 8 μl of pure water, Highly significant correlation ( R 2 > 0.99), with slope values within an acceptable range (between −3.1 and −3.6) ( Table 1 ).

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