Ultimately, the most active mutant obtained in this study exhibited a k cat / K m value of 24.43 M −1 s −1 , representing a highly significant improvement compared to the previously reported data (3.54 M −1 s −1 ) (Li et al. 2017 ).
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Engineering of 10-Deacetylbaccatin III-10-β-O-Acetyltransferase From Taxus Species for Efficient Acetylating Non-Natural Substrates Into Taxol in Nicotiana benthamiana.
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