Comparative transcriptome analysis reveals stress-response pathways as key drivers of nitrogen use efficiency in tobacco under low-nitrogen conditions.

In leaves, all examined ER-related genes (9/9) showed highly significant cultivar effects ( P < 0.01), and six genes exhibited significant growth-stage effects ( P < 0.05), with most genes displaying progressive up-regulation from 50 to 100 DAT in Hongda, whereas expression levels in K326 remained consistently low or unchanged.

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